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Anderson, K.E. and J.H. Waite, 1997. A Major Zebra Mussel Byssal Precursor Protein is Part of a Polymorphic Family of Proteins. University of Delaware Reprinted from American Zoology (1997) 37(5): 125A A Major Zebra Mussel Byssal Precursor Protein is Part of a Polymorphic Family of ProteinsRT-PCR of zebra mussel (Dreissena polymorpha) foot tissue total RNA has been successfully used to amplify three cDNA variants coding for byssal precursor protein: Dreissena polymorpha foot protein 1 (Dpfp1). These results suggest that like many of its marine counterparts. Dpfpl is a member of a polymorphic family of byssal structural proteins. Analysis of sequence data indicates that the variants differ primarily in the number of timer a 13 amino acid consensus motif is repeated in the C-terminal portion of this protein. A smaller seven amino acid consensus motif confined to the N-terminal portion of Dpfpl is not similarly affected in these variants. The above-mentioned modification produce variants with pls ranging from 5.0-5.6. an observation consistent with previously noted electrophoretic heterogeneity during isoelectric focusing of native Dpfpl (Mar. Biol. Biotech 2267). None of the three variants contain deletions of putative O-glycosylation sites suggesting that glycosyluion is important in maintaining the adhesive and or cohesive properties of Dpfp. NOAA is gratefully acknowled for supporting this work via Grant Award #NA46RG0480 Contact: K.E. Anderson, Chemistry and Biochemistry, University of
Delaware, Newark, DE 19716 anderson@harpo.duch.udel.edu |