Flynn, K., P. Schoff, and J. Holy, 1998. Localization of Ruffe Testicular Antigens by a Panel of Antibodies, University of Minnesota.

Reprinted from Journal of Great Lakes Research (1998) 24(2): 379-382

 Localization of Ruffe Testicular Antigens by a Panel of Antibodies

A panel of antibodies was produced using ruffe sperm and testicular tissue as the immunogen. Eight hybridoma cell lines were selected by screening formaldehyde-fixed cryostat sections of testes. Based on immunolabeling patterns, five groups of testicular antigens were identified: 1) "sperm surface" (entire surface of sperm labeled), 2) "punctate" (a few foci on each sperm head labeled), 3) "painted" (filamentous material within the spermatocyst labeled), 4) "lumenal" (particulate material within the spermatocyst labeled), and 5) "CT" (testicular connective tissue is labeled). The hybridomas that produced antibodies recognizing antigens located on the surface of ruffe sperm are of particular interest because of the possibility that they may be identifying molecules involved in sperm-egg binding and fertilization. 

Contact: Kevin Flynn, University of Minnesota, Department of Anatomy and Cell Biology, School of Medicine, Duluth, MN 55812
Keywords: Ruffe, Basic_biology, Prevention
Product Type: Research, Basic_biology
User Type: General